That's a good question. The issue is that the timers are graduated in microseconds, and MultiShot uses 5-25us IIRC. We would have to use timers graduated in shorter intervals than microseconds and I don't know if the hardware supports it, and if it does, what performance hits it would cause, and also the compatibility issues with 1us timer res.
If I ruled the world, I would say it's not worth it. DShot would be better.
Either LOS or FPV would be fine as long as it has audio. I don't think you will be able to tell visually or from feel, but it might be possible to hear the ESC's switching back and forth between say 11us and 12us in a motionless hover.